GUIDES: sgRNA design for loss-of-function screens

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

investigating the feasibility of a proposed model for geometric design of deployable arch structures

deployable scissor type structures are composed of the so-called scissor-like elements (sles), which are connected to each other at an intermediate point through a pivotal connection and allow them to be folded into a compact bundle for storage or transport. several sles are connected to each other in order to form units with regular polygonal plan views. the sides and radii of the polygons are...

Sequence determinants of improved CRISPR sgRNA design.

The CRISPR/Cas9 system has revolutionized mammalian somatic cell genetics. Genome-wide functional screens using CRISPR/Cas9-mediated knockout or dCas9 fusion-mediated inhibition/activation (CRISPRi/a) are powerful techniques for discovering phenotype-associated gene function. We systematically assessed the DNA sequence features that contribute to single guide RNA (sgRNA) efficiency in CRISPR-ba...

متن کامل

Bi-objective Economic statistical design of the joint Xbar and S charts incorporating Taguchi loss function

In this research, we propose a bi-objective model for the economic-statistical design of the X-bar and S control charts. The model minimizes out-of-control average time to signal as well as minimizing mean hourly loss-cost where it incorporates the Taguchi loss function. Statistical constraint is considered in the model to achieve desired in-control time to signal. A non-dominated sort...

متن کامل

Disorder guides protein function.

Cellular function requires biomolecules to undergo dynamic transitions that include folding, conformational rearrangements, and large-scale assembly. The result is a highly interdependent network of processes that is maintained by a balance of thermodynamic and kinetic factors. In molecular machines, each constituent biopolymer (i.e., a chain of residues) first folds to a lowenergy configuratio...

متن کامل

simulation and design of electronic processing circuit for restaurants e-procurement system

the poor orientation of the restaurants toward the information technology has yet many unsolved issues in regards to the customers. one of these problems which lead the appeal list of later, and have a negative impact on the prestige of the restaurant is the case when the later does not respond on time to the customers’ needs, and which causes their dissatisfaction. this issue is really sensiti...

15 صفحه اول

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Nature Methods

سال: 2017

ISSN: 1548-7091,1548-7105

DOI: 10.1038/nmeth.4423